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Observation 929

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Species Drosophila melanogaster
Strain w1118
Sex female
Temperature 25
Lifespan 60
Reference Lifespan 60
Lifespan Change
Lifespan Measure median
Lifespan Effect none
Citation Xie X, Hu J, Liu X, Qin H, Percival-Smith A, Rao Y, Li SS. (2010). NIP/DuoxA is essential for Drosophila embryonic development and regulates oxidative stress response. Int. J. Biol. Sci. [pubmed]
RNAi knock-down of NIP/DuoxA shortened life span when animals were maintained at 29 degrees C but had not effect on life span at 25 degrees C. RNAi knockdown was accomplished by GAL4 inducible UAS-RNAi transgenes. daG32-GAL4 driven, ubiquitous RNAi-mediated silencing of nip led to profound abnormality in pre-adult development, crinkled wing and markedly reduced lifespan at 29°C. Compared to wild type flies, da-GAL4 induced nip-RNAi transgenic flies exhibited significantly reduced ability to survive under oxidative stress and displayed impaired mitochondrial aconitase function.

Gene Interventions:

Symbol Allele Allele Type NCBI Gene ID More
NIP RNAi knockdown