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Observation 928

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Species Drosophila melanogaster
Strain w1118
Sex female
Temperature 29
Lifespan 10
Reference Lifespan 40
Lifespan Change -75%
Lifespan Measure median
Lifespan Effect decreased
Significance significant
Citation Xie X, Hu J, Liu X, Qin H, Percival-Smith A, Rao Y, Li SS. (2010). NIP/DuoxA is essential for Drosophila embryonic development and regulates oxidative stress response. Int. J. Biol. Sci. [pubmed]
RNAi knock-down of NIP/DuoxA shortened life span when animals were maintained at 29 degrees C but had not effect on life span at 25 degrees C. RNAi knockdown was accomplished by GAL4 inducible UAS-RNAi transgenes. daG32-GAL4 driven, ubiquitous RNAi-mediated silencing of nip led to profound abnormality in pre-adult development, crinkled wing and markedly reduced lifespan at 29°C. Compared to wild type flies, da-GAL4 induced nip-RNAi transgenic flies exhibited significantly reduced ability to survive under oxidative stress and displayed impaired mitochondrial aconitase function.

Gene Interventions:

Symbol Allele Allele Type NCBI Gene ID More
NIP RNAi knockdown