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Observation 3949

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Species Escherichia coli
Strain BW25113
Temperature 37
Lifespan 7 days
Reference Lifespan 1.5 days
Lifespan Change 366.67%
Lifespan Measure median
Lifespan Effect increased
Significance significant
Citation Gonidakis S, Finkel SE, Longo VD. (2010). Genome-wide screen identifies Escherichia coli TCA-cycle-related mutants with extended chronological lifespan dependent on acetate metabolism and the hypoxia-inducible transcription factor ArcA. Aging Cell 9: 868-81. [pubmed]
E. coli strains lacking tricarboxylic acid (TCA)-cycle-related genes exhibit longer stationary-phase survival and increased resistance to heat stress compared to wild-type. Extended lifespan in the sdhA mutant, lacking subunit A of succinate dehydrogenase, is associated with the reduced production of superoxide and increased stress resistance. On the other hand, the longer lifespan of the lipoic acid synthase mutant (lipA) is associated with reduced oxygen consumption and requires the acetate-producing enzyme pyruvate oxidase, as well as acetyl-CoA synthetase, the enzyme that converts extracellular acetate to acetyl-CoA. The hypoxia-inducible transcription factorArcA, acting independently of acetate metabolism, is also required for maximum lifespan extension in the lipA and lpdA mutants, indicating that these mutations promote entry into a mode normally associated with a low-oxygen environment.

Gene Interventions:

Symbol Allele Allele Type NCBI Gene ID More
atpC deletion / null