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Observation 2222

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Species Caenorhabditis elegans
Strain N2
Sex hermaphrodite
Temperature 22.5
Lifespan 71.8 days
Reference Lifespan 16.3 days
Lifespan Change 340.49%
Lifespan Measure median
Lifespan Effect increased
Significance significant
Citation Gems D, Sutton AJ, Sundermeyer ML, Albert PS, King KV, Edgley ML, Larsen PL, Riddle DL. (1998). Two pleiotropic classes of daf-2 mutation affect larval arrest, adult behavior, reproduction and longevity in Caenorhabditis ele. Genetics 150: 129-55. [pubmed]
Details
15 temperature-sensitive and 1 nonconditional daf-2 allele were characterized to investigate the extent of daf-2 mutant defects and to examine whether specific mutant traits correlate with each other. The greatest longevity seen in daf-2 mutant adults was approximately three times that of wild type. The temperature-sensitive daf-2 mutants fell into two overlapping classes, including eight class 1 mutants, which are Daf-c, Age, and Itt, and exhibit low levels of L1 arrest at 25.5 degrees. Seven class 2 mutants also exhibit the class 1 defects as well as some or all of the following: reduced adult motility, abnormal adult body and gonad morphology, high levels of embryonic and L1 arrest, production of progeny late in life, and reduced brood size. The strengths of the Daf-c, Age, and Itt phenotypes largely correlated with each other but not with the strength of class 2-specific defects. This suggests that the DAF-2 receptor is bifunctional.

Gene Interventions:

Symbol Allele Allele Type NCBI Gene ID More
daf-12 m20 unknown
daf-2 e1391 unknown