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Observation 2109

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Species Saccharomyces cerevisiae
Strain W303AR5
Mating Type matalpha
Temperature 30
Lifespan 22 days
Reference Lifespan 4 days
Lifespan Change 450%
Lifespan Measure median
Lifespan Effect increased
Significance significant
Citation Burtner CR, Murakami CJ, Kennedy BK, Kaeberlein M. (2009). A molecular mechanism of chronological aging in yeast. Cell Cycle 8: 1256-70. [pubmed]
Buffering the culture medium to pH 6 with citrate-phosphate or MES buffers increases the chronological life span of cells grown in synthetic defined medium.

Environment Interventions

Type Body
pH 6 buffering Media was buffered to pH 6 in 0.1 M citrate-phosphate buffer