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Observation 117

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Species Saccharomyces cerevisiae
Strain PSY316
Reference Lifespan
Lifespan Change
Lifespan Measure
Lifespan Effect increased
Significance significant
Citation Lin SJ, Kaeberlein M, Andalis AA, Sturtz LA, Defossez PA, Culotta VC, Fink GR, Guarente L. (2002). Calorie restriction extends Saccharomyces cerevisiae lifespan by increasing respiration.. Nature 418: 344-8. [pubmed]

Overexpression of HAP4 from the ADH1 promoter extends life-span approximately 40% under growth conditions favoring fermentation (2% glucose).

Other phenotypes: Overexpression of HAP4 combined with calorie restriction (CR) fails to further extend life-span. Like CR, life-span extension by HAP4 requires SIR2. HAP4 overexpressing cells demonstrate a transcriptional response resembling cells undergoing diauxic shift, consume more oxygen than wild type cells, and show increased Sir2p-dependent transcriptional silencing at telomeres and rDNA [pubmed].

Gene Interventions:

Symbol Allele Allele Type NCBI Gene ID More
HAP4 over-expression 853751 View