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Observation 113

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Species Homo sapiens
Strain MRC-5 lung fibroblasts
Cell Type cell culture
Reference Lifespan
Lifespan Change
Lifespan Measure
Lifespan Effect increased
Significance significant
Citation Wadhwa R, Kaul SC, Ikawa Y, Sugimoto Y. (1993). Identification of a novel member of mouse hsp70 family. Its association with cellular mortal phenotype.. J. Biol. Chem. 268: 6615-21. [pubmed]

Transfection of normal human fiboblasts with human or murine Mot-2 overexpression vectors led to an increase in the number of population doublings the cells could sustain before senescing (percent increase varying from 32% to 60%, depending on the exact construct used).

Other phenotypes: Mot-2 is an hsp70 family member found to be differentially distributed and/or translated in normal vs. transformed cells [pubmed]. Mot-2 overexpressing cells exhibited a reduction in p53 transcriptional activation, as measured by expression from vectors containing either luciferase or beta-galactosidase driven by p53 binding sites [pubmed]. Furthermore, the transfected cells retained a youthful morphology longer than the control cells, and there was a delay in appearance of senescence associated beta-galactosidase activity [pubmed].

Gene Interventions:

Symbol Allele Allele Type NCBI Gene ID More
Mot-2 over-expression